Journal: Journal for Immunotherapy of Cancer

Article Title: Pharmacologic targeting of the dopamine D2 receptor impacts the efficacy of immune checkpoint blockade in melanoma

doi: 10.1136/jitc-2025-014080

Figure Lengend Snippet: MCP enhances T cell activation and modulates macrophage polarization. ( A ) CD44 expression measured by flow cytometry as MFI on OT-I CD8+ T cells activated with 50 ng/mL SIINFEKL, SIIQFEKL, SIITFEKL, or SIIVFEKL with or without 100 µg/mL MCP for 48 hours in vitro. ( B, C, D ) CD25, CD69, PD-1 expression. IL-12β ( E ) and NOS2 ( F ) expression measured by quantitative reverse transcription PCR in bone marrow-derived M0 macrophages polarized to the M1 phenotype with or without 100 µg/mL MCP for 24 hours in vitro. ( G ) Arg1 expression in M0 macrophages polarized to the M2 phenotype with or without MCP for 24 hours in vitro. P values determined by Student’s t-test. *p<0.05; **p<0.01. IL, interleukin; MCP, metoclopramide; MFI, mean fluorescence intensity; PD-1, programmed cell death protein-1.

Article Snippet: Primers include murine Gapdh (Mm99999915_g1), B2m (Mm00437762_m1), Arg1 (Mm00475988_m1), Nos2 (Mm00440502_m1), Il-12β (Mm01288989_m1), and PRL (Mm00599950_m1).

Techniques: Activation Assay, Expressing, Flow Cytometry, In Vitro, Reverse Transcription, Derivative Assay, Fluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Refined in vivo model for bone regeneration: insights into scaffold architecture and porosity

doi: 10.3389/fbioe.2026.1725958

Figure Lengend Snippet: ARG1 immunohistochemical analysis at 2 weeks (a,b, b1,b2 ) and 4 weeks (c, d, d1,d2 ) within 3D-CPC scaffolds (250 and 500 µm) neutrophils and macrophages M2 staining. Masson–Goldner staining at 2 and 4 weeks (a,c) showed bone formation (green) within pores of 250 µm (left) and 500 µm (right). The same samples were then immunostained for ARG1 (b,d) and violin plots of ARG1-positive cell distribution were superimposed. The blue and red curves indicate the spatial distribution of ARG1-positive cells within scaffolds possessing pore diameters of 250 µm and 500 μm, respectively, while the horizontal profile reflects the probability density of cell occurrence across distances (smoothed by a kernel density estimator). (n = 8). At 2 weeks, ARG1-positive cells (black arrows) were mainly detected at the upper region of 250 µm scaffolds, close to the migration front, whereas in 500 µm scaffolds staining was more pronounced in central osteoid-rich areas. By 4 weeks, ARG1 expression shifted upward in both scaffold types, with M2 macrophages and neutrophils present within mineralizing tissue. In 500 µm scaffolds, this zone was located slightly deeper than in the 250 µm scaffolds. The scaffold is tagged by a S. Black bars 500 μm, White bars 100 µm.

Article Snippet: Quantitative real-time PCR (qRT-PCR) was conducted using TaqMan primers (ThermoFisher) targeting inflammatory, regenerative, and autophagy-related genes, includingIL1β (Oc03823250_s1), IL6 (Oc04097053_m1), TNFα (Oc03397715_m1), CXCL12 (Oc06731048_m1), MPO (Oc06780710_m1), ALOX15 (Oc03823548_s1), CCL2 (Oc03823583_s1), ARG1 (Oc03397218_m1), MAP1LC3C (Oc06779063_s1), ATG5 (Oc06724789_m1), ATG7 (Oc06724789_m1), WIPI1 (Oc06736980_m1), ULK1 (Oc06733082_g1), TLR4 (Oc03398504_m1), CD36 (Oc03395926_m1), PECAM1 (Oc06726473_m1), IL10 (Oc03396940_m1), SLC15A2 (Oc03398448), MMP12 (Oc03398612_m1), CXCL10 (Oc06781608_g1), FUT4 (Oc06805515_s1).

Techniques: Immunohistochemical staining, Staining, Migration, Expressing

Journal: Frontiers in Neurology

Article Title: Early Microglial Activation Following Closed-Head Concussive Injury Is Dominated by Pro-Inflammatory M-1 Type

doi: 10.3389/fneur.2018.00964

Figure Lengend Snippet: Gene table for inflammation profile array genes and their TaqMan IDs.

Article Snippet: Rn00691090_m1 , Rn01464736_g1 , Rn00584362_m1 , Rn00586403_m1 , Rn01483988_g1 , Rn01762214_m1 , Rn01412404_m1 , Rn00567818_m1 , Rn00671924_m1 , Rn00709368_m1 , Rn01525859_g1.

Techniques:

Journal: American Journal of Physiology - Renal Physiology

Article Title: Subclinical kidney injury induced by repeated cisplatin administration results in progressive chronic kidney disease

doi: 10.1152/ajprenal.00636.2017

Figure Lengend Snippet: Total macrophages and macrophage subtypes during and after repeated cisplatin (CDDP) treatment. Eight-week-old FVB mice were treated with either saline vehicle or CDDP (7 mg/kg) once per week for 2 wk and euthanized 3 days after dose 2 (D2). Another group was treated with either saline vehicle or CDDP (7 mg/kg) once per week for 4 wk and euthanized 3 days after dose 4 (D4), or allowed to age for 6 mo following treatment (6 mo post-CDDP). F4/80 IHC staining for total macrophages (A). mRNA levels of inducible nitric oxide synthase (iNos) (B) and arginase-1 (Arg-1) (C) measured in the kidney cortex via qRT-PCR. Data are expressed as means ± SE; n = 5–10.

Article Snippet: The following TAQman assays (Life Technologies) were used: Tnfα (Mm00443258_m1), Il-6 (Mm00446190_m1), Cxcl1 (Mm04207460_m1), Mcp-1 (Mm00441242_m1), Cdkn1a (Mm01303209_m1), Ctgf (Mm01192932_m1), Col1α1 (Mm00801666_m1), Arg-1 (Mm00475991_m1), and B2m (Mm00437762_m1).

Techniques: Saline, Immunohistochemistry, Quantitative RT-PCR

Journal: Obesity (Silver Spring, Md.)

Article Title: Adipose tissue remodeling in a novel domestic porcine model of diet-induced obesity

doi: 10.1002/oby.20971

Figure Lengend Snippet: In subcutaneous adipose tissue, the number of M1 macrophages similarly increased in obese pigs from 8 to 16 weeks, while the number of M2 macrophages only increased in obese pigs at 16 weeks compared with 8 weeks and with lean (A). In pericardial adipose tissue, only M1 macrophages increased in obese compared with lean (B). Macrophage mRNA (CD68) and both M1 (CD 86, CD11c) and M2 (Arginase-1, mannose-receptor) markers increased only in pericardial adipose tissue, while TNF-α mRNA increased in both subcutaneous and pericardial fat (C-D). *p<0.05 vs. lean. †p<0.05 vs. 12wks

Article Snippet: Taqman assays included the followings: iNOS (Ss03374608), CD86 (Ss03394398), mannose-receptor (Ss03373693), CD11c (AJ1RVEU), TNF-a (Ss03391318), IL-6 (Ss03384604), CD-68 (AJWR2PY), arginase-1 (Ss03391398) and GAPDH (Ss03374854, for internal control), all from Life Technologies.

Techniques: